A new liver function turbidity test.
نویسنده
چکیده
The various liver flocculation tests proposed in recent years by several investigators cannot be considered as specific, hence clinicians tend to use many of them simultaneously. Obviously, the simplicity and the rapidity with which any one of these tests can be performed are essential advantages. The following new liver function turbidity test is simple and rapid, and is more sensitive than the other known tests. In 1949 for the first time it was observed that che addition of diethylbarbituric acid to human blood serum in a test-tube produced a characteristic turbidity which was dense in serum from patients with hepatic diseases. Later it was determined that a saturated solution of diethylbarbituric acid produced turbidity with serum both from patients with hepatic diseases and from healthy individuals. However, with a solution of 0.44 g. per 1,000 ml. of distilled water, a dense turbidity was obtained with serum from clinical cases of liver damage and slight or no turbidity in normals. The addition of 0.05 ml. of blood serum taken from clinically positive cases to 4 ml. of the diluted solution of diethylbarbituric acid produced almost immediately a dense turbidity, which, at the peak of the reaction 20 minutes later, was compared with the nephelometric scale of Kingsbury-Clark. During this primary procedure it was observed that when the mixture of serum and diethylbarbituric acid in the test-tube was heated for one minute in a water-bath the turbidity obtained was dense and more stable than without heating. It remained unchanged for over two weeks.
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 6 4 شماره
صفحات -
تاریخ انتشار 1953